Anti-Bcl-2 Monoclonal Antibody for WB - P10415 / P49950

Core Product Specifications and Parameters

Biological Background: Bcl-2 Function and Localization

• Apoptosis regulator Bcl-2 (also known as PPP1R50) is encoded by the BCL2 gene and undergoes alternative splicing.
• Bcl-2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Related references: PMID:1508712 PMID:8183370
• It regulates cell death by controlling mitochondrial membrane permeability, functioning in a feedback loop with caspases, and inhibiting caspase activity by preventing cytochrome c release and binding APAF-1. Related references: PMID:11368354
• Bcl-2 also acts as an inhibitor of autophagy through interaction with BECN1 and AMBRA1 during non-starvation conditions. Related references: PMID:18570871 PMID:20889974 PMID:21358617
• It may attenuate inflammation by impairing NLRP1-inflammasome activation, thereby reducing CASP1 activation and IL1B release. Related references: PMID:17418785
• Bcl-2 localizes to the mitochondrial outer membrane, nuclear membrane, endoplasmic reticulum membrane, and cytoplasm.
• It is broadly expressed in a variety of tissues.
• Bcl-2 is a phosphoprotein subject to ubiquitin-like conjugation and is associated with chromosomal rearrangements in diseases such as lymphoma.

Experimental Guidance and Technical Tips

• This recombinant monoclonal antibody was generated using a synthetic peptide corresponding to human/rat Bcl-2; it may detect conserved epitopes across species.
• Validated applications include Western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA.
• The antibody cross-reacts with human, mouse, and rat Bcl-2, making it suitable for multi-species studies.
• For optimal results, titrate the antibody and validate in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to a comprehensive portfolio of IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. Our offerings include optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets like Bcl-2. Partner with us to streamline your development of apoptosis and oncology diagnostic solutions.