Anti-BCKDK Polyclonal Antibody for WB, IF/ICC, ELISA - O14874

Core Product Specifications and Parameters

Biological Background: BCKDK Function and Localization

• BCKDK is a serine/threonine-protein kinase that serves as a key metabolic regulator, forming a functional pair with the phosphatase PPM1K to coordinate branched-chain amino acid (BCAA) catabolism with glucose and lipid metabolism. Related references: PMID:24449431 PMID:29779826 PMID:37558654
• It phosphorylates and inactivates the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex by targeting the BCKDHA subunit at Ser-337, thereby interrupting substrate channeling and downregulating BCAA degradation.
• Additionally, BCKDK phosphorylates cytosolic ATP-citrate lyase (ACLY) at Ser-455 in response to carbohydrate abundance, promoting lipogenesis and gluconeogenesis during the fasting-to-feeding transition. Related references: PMID:29779826
• The protein localizes to the mitochondrial matrix.
• BCKDK is ubiquitously expressed across human tissues.
• UniProt annotations associate BCKDK with autism spectrum disorder, epilepsy, and intellectual disability.
• The kinase recognizes a distinct SxxE/D phosphosite motif.

Experimental Guidance and Technical Tips

• The immunogen (aa 153-412) lies within the kinase domain; consider sequence alignment to predict cross-reactivity across species and isoforms.
• For WB, a band near the predicted 46 kDa is expected; always include positive (e.g., human/rat tissue lysates) and negative controls to confirm specificity.
• In IF/ICC, mitochondrial staining patterns may be observed; co-staining with organelle markers is recommended for validation.
• For ELISA, titrate both coating antigen and antibody concentrations to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes comprehensive access to IVD raw materials—from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents. For studies on BCAA metabolism and macronutrient regulation, we facilitate sourcing of rare targets like BCKDK, supported by integrated technical services spanning concept to clinic. Let our supply chain expertise accelerate your next metabolic assay development.