Anti-BCKDK Monoclonal Antibody for WB, ELISA - O14874

Core Product Specifications and Parameters

Biological Background: BCKDK Function and Localization

• BCKDK (Branched-chain alpha-ketoacid dehydrogenase kinase) is a serine/threonine-protein kinase that forms a functional pair with the phosphatase PPM1K, serving as a metabolic regulatory node coordinating branched-chain amino acids (BCAAs) with glucose and lipid metabolism. Related references: PMID:24449431 PMID:29779826 PMID:37558654
• It phosphorylates the BCKDHA subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex on Ser-337, leading to inactivation of the complex and suppression of BCAA catabolism. Related references: PMID:29779826 PMID:37558654
• BCKDK also phosphorylates cytosolic ACLY (ATP citrate lyase) on Ser-455 in response to carbohydrate abundance, enhancing lipogenesis and glucogenesis during the fasting-to-feeding transition. Related references: PMID:29779826
• It recognizes phosphosite motifs with the canonical sequence SxxE/D. Related references: PMID:29779826
• Located in the mitochondrial matrix, BCKDK associates with the E2 component of the BCKDH complex.
• Expressed ubiquitously across tissues.
• UniProt keywords associate BCKDK with autism spectrum disorder, epilepsy, and intellectual disability, suggesting potential disease relevance.
• Post-translational modifications include acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100) of human BCKDK; for WB analysis, consider whether the epitope remains accessible in your specific sample preparation.
• Cross-reactivity with mouse and rat makes this antibody a candidate for translational metabolic studies in rodent models.
• For ELISA applications, validate the antibody in your target sample matrix and optimize blocking and washing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to metabolic research. We support studies on key enzymes like BCKDK, offering reliable reagents for BCAA metabolism and mitochondrial kinase investigations. From concept to clinic, our technical services and consulting accelerate your diagnostic development.