Anti-BCDIN3D Rabbit Polyclonal Antibody for WB, ELISA - Q7Z5W3

Core Product Specifications and Parameters

Biological Background: BCDIN3D Function and Localization

• BCDIN3D (RNA 5'-monophosphate methyltransferase) is a methyltransferase that specifically monomethylates the 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme to protect it from DICER1 cleavage.
  Related references: PMID:28119416, PMID:31329584, PMID:31919512
• It also methylates the 5' monophosphate of a subset of pre-miRNAs, negatively regulating miRNA processing by reducing DICER1-mediated processing.
  Related references: PMID:23063121, PMID:28119416
• The protein is localized in the cytoplasm.
• The calculated molecular weight is 33.2 kDa, consistent with the observed 33 kDa.
• Keywords associated with BCDIN3D include 3D-structure, Methyltransferase, S-adenosyl-L-methionine, Transferase.
• Note: No tissue specificity data is available from the UniProt record.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 1-292, the full-length BCDIN3D; this should provide broad epitope coverage for Western blot detection. Consider verifying the antibody’s performance using a positive control such as lysates from human or mouse cell lines expressing BCDIN3D.
• For ELISA applications, use the recombinant immunogen as a standard to optimize antibody concentration and confirm binding specificity.
• Cross-reactivity is indicated for human and mouse; validate the antibody in your specific sample type (e.g., cell lysate, tissue extract) to ensure reactivity.
• The predicted protein weight is ~33 kDa. When running WB, ensure sufficient gel resolution in the 25–40 kDa range and use appropriate molecular weight markers.
• Shipping on ice bags; upon receipt, aliquot and store at appropriate conditions (e.g., -20°C) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

As a global one-stop IVD raw material partner, CamelBio supplies diagnostic developers and researchers with premium antibodies like this anti-BCDIN3D rabbit polyclonal antibody, supporting RNA modification and epitranscriptomics investigations. Our portfolio extends to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary bulk reagents, streamlining your assay development from concept to clinic. For rare or custom targets, we offer sourcing services to meet your unique raw material needs.