Anti-BCAT2 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - O15382

Core Product Specifications and Parameters

Biological Background: BCAT2 Function and Localization

• BCAT2 (branched chain amino acid transaminase 2), also known as placental protein 18 (PP18), is a mitochondrial aminotransferase.
• Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Related references: PMID:17050531, PMID:25653144, PMID:8702755
• Branched chain amino acid catabolism provides acetyl-CoA for lipid synthesis during adipocyte differentiation and may regulate adipose tissue browning by modulating PRDM16 acetylation.
• The enzyme is ubiquitously expressed and localizes to the mitochondrion.
• BCAT2 is a pyridoxal phosphate-dependent enzyme belonging to the class-IV aminotransferase family; post-translational modifications include acetylation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 28 – 392 of human BCAT2, representing nearly the full-length protein, which supports high target specificity.
• In western blot, the predicted mitochondrial form migrates at ~44 kDa; additional bands (e.g., ~33 kDa) may reflect alternative isoforms or processing.
• The antibody has been reported for WB, IF-P, IHC-P, and ELISA; for each application, titrate and validate conditions in the relevant sample system.
• Cross-reactivity with mouse and rat extends utility to rodent models, but confirm band patterns in your model of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers by streamlining access to premium IVD raw materials. Our portfolio includes this anti-BCAT2 monoclonal antibody alongside validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, all supported by end-to-end technical guidance. Whether you are developing assays for branched-chain amino acid metabolism or other disease areas, CamelBio is your trusted sourcing partner.