Anti-BCAT2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15382

Core Product Specifications and Parameters

Biological Background: BCAT2 Function and Localization

• BCAT2 (Branched-chain-amino-acid aminotransferase, mitochondrial) is a pyridoxal phosphate-dependent enzyme that initiates the catabolism of branched-chain amino acids (leucine, isoleucine, valine), a critical step in energy metabolism. Related references: PMID:17050531, PMID:25653144, PMID:8702755

• In adipocytes, BCAT2-generated acetyl-CoA feeds into lipogenesis and modulates differentiation and browning processes via EP300/p300-mediated acetylation of PRDM16.

• The enzyme resides in the mitochondrial matrix, aligning with its role in amino acid degradation and the provision of metabolic intermediates for the TCA cycle and lipid synthesis.

• BCAT2 is ubiquitously expressed, indicating a fundamental housekeeping function in all cell types.

• Alternative splicing of the BCAT2 transcript gives rise to multiple isoforms, potentially expanding functional versatility.

• Post-translational modifications, including acetylation, fine-tune BCAT2 activity, while its association with disease variants underscores its clinical significance in metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen, covering amino acids 28-392 of human BCAT2, includes the majority of the mature protein; thus, the antibody may recognize multiple isoforms and native conformations.
• For Western blotting, expect a band near 44 kDa; validate with mitochondrial lysates from human, mouse, or rat tissues as positive controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), mitochondrial staining should be confirmed, and antigen retrieval conditions optimized for each sample type.
• For ELISA, cross-reactivity with other BCAT family members or species-specific variants should be assessed using appropriate recombinant proteins or lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement by connecting diagnostic manufacturers and research teams with high-quality antibodies and ancillary reagents. This anti-BCAT2 polyclonal antibody supports metabolic and mitochondrial research applications. For your broader project needs, we offer validated antibody pairs, optimized recombinant proteins, and custom sourcing of rare-target materials—helping you secure the right reagents at every stage of development.