Anti-BCAT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P54687

Core Product Specifications and Parameters

Biological Background: BCAT1 Function and Localization

• BCAT1 (Branched-chain-amino-acid aminotransferase, cytosolic) is a pyridoxal phosphate-dependent enzyme that belongs to the class-IV aminotransferase family.
• It catalyzes the first step in the catabolism of the essential branched-chain amino acids (BCAAs) leucine, isoleucine, and valine, converting them to their respective α-keto acids.
• The gene BCAT1 (also known as BCT1, ECA39) encodes this cytosolic isoform; alternative splicing produces multiple transcript variants.
• BCAT1 is localized predominantly in the cytoplasm.
• During embryogenesis, BCAT1 is expressed in the brain and kidney; it is overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
• The enzyme is subject to post-translational acetylation and is linked to amino acid biosynthesis and lipid metabolism.
• As a key metabolic enzyme, BCAT1 is involved in branched-chain amino acid metabolism, which is important in cancer and tissue homeostasis.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment corresponding to amino acids 20-190 of human BCAT1, which may target the N-terminal region conserved across species, supporting the observed cross-reactivity with human, mouse, and rat.
• For Western blot (WB), the predicted molecular weight is approximately 43 kDa; consider validating with relevant positive and negative controls to confirm specificity.
• In immunofluorescence/immunocytochemistry (IF/ICC), BCAT1 localization is expected to be cytoplasmic; use appropriate fixation and permeabilization protocols for optimal detection.
• Some targets may require further dilution adjustments; consider optimizing antibody concentration in your experimental system.
• This polyclonal antibody may be suitable for detecting both endogenous and overexpressed BCAT1; consider testing in both wild-type and knockdown/overexpression models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our BCAT1 Rabbit pAb supports research into branched-chain amino acid metabolism and MYC-driven tumor biology. With end-to-end technical and supply chain services, we help accelerate your projects from concept to clinic.