Anti-BCAS3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q9H6U6

Core Product Specifications and Parameters

Biological Background: BCAS3 Function and Localization

• BCAS3 (Breast carcinoma-amplified sequence 3), also known as GAOB1, is a microtubule-associated cell migration factor that contains WD repeats and acts as a proto-oncogene.
• Plays a pivotal role in angiogenesis by regulating cell polarity and directional endothelial cell migration, mediating activation and recruitment of CDC42 and reorganization of the actin cytoskeleton at the leading edge.
• Functions synergistically with PELP1 as a transcriptional coactivator of estrogen receptor-responsive genes, stimulating histone acetyltransferase activity and binding to chromatin.
• Participates in autophagy regulation; in complex with PHAF1, it associates with preautophagosomal structures and likely binds phosphatidylinositol 3-phosphate (PtdIns3P) to mediate recruitment during both non-selective and selective autophagy. Related references: PMID:33499712
• Localizes to the nucleus, cytoplasm, cytoskeleton, and preautophagosomal structures, consistent with its roles in transcription, cytoskeletal dynamics, and autophagy.
• Broadly expressed in tissues including stomach, liver, lung, kidney, prostate, testis, thyroid, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocytes, and mammary epithelial cells; highly expressed in breast cancers and glioma cell lines.
• Post-translationally modified by acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), which may regulate its activity in signaling and protein stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 674–913 of human BCAS3, a region that may be conserved across species; consider validating cross-reactivity in your specific mouse and rat sample systems if needed.
• For western blot (WB), the predicted molecular weight is approximately 101 kDa; use appropriate gel percentage and transfer conditions for efficient detection.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize fixation, antigen retrieval, and permeabilization protocols to ensure proper epitope exposure and subcellular localization.
• This antibody’s polyclonal nature may yield robust signals in immunoprecipitation (IP) and ELISA; titrate antibody concentrations and include relevant controls to confirm specificity.
• Given BCAS3’s involvement in autophagy, consider using appropriate autophagy inducers/inhibitors when designing functional assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined sourcing platform for premium IVD raw materials, including validated polyclonal and monoclonal antibodies. This anti-BCAS3 antibody supports critical areas such as angiogenesis, cancer biology, and autophagy research. Beyond standard catalog products, we provide rare target sourcing, bulk reagent supply, and tailored technical consulting to accelerate your assay development from concept to clinic.