Anti-BCAR1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P56945

Core Product Specifications and Parameters

Biological Background: BCAR1 Function and Localization

• BCAR1 (Breast cancer anti-estrogen resistance protein 1, also known as p130cas, CAS, CASS1, CRKAS) is a docking protein and a member of the Cas scaffolding protein family. It exists in multiple isoforms due to alternative splicing.
• BCAR1 plays a central coordinating role in tyrosine kinase-based signaling pathways that regulate cell adhesion. It is implicated in the induction of cell migration and cell branching. Related references: PMID:12432078, PMID:12832404, PMID:17038317
• It is involved in the BCAR3-mediated inhibition of TGF-beta signaling (By similarity).
• Subcellularly, BCAR1 localizes to cell junctions, focal adhesions, the cytoplasm, and cell projections such as axons.
• As a phosphoprotein that undergoes acetylation, BCAR1 contains both an SH3 domain and SH3-binding motifs, facilitating protein-protein interactions essential for signal transduction.
• BCAR1 is widely expressed, with abundant expression in the testis and lower levels in the liver, thymus, and peripheral blood leukocytes. It is also expressed in B-cells at the protein level.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 495–612, a region within the central domain of BCAR1, which may detect multiple isoforms if the sequence is conserved.
• For Western blot, expect a band near 93 kDa; consider using cell lines with established BCAR1 expression (e.g., breast cancer lines) and include phosphatase inhibitors to preserve phosphorylation.
• In IHC-P, antigen retrieval conditions should be empirically optimized; use testis sections as a positive control due to high BCAR1 expression.
• For IF/ICC, fixation and permeabilization protocols need optimization to visualize BCAR1 at focal adhesions and in the cytoplasm.
• Cross-reactivity with mouse and rat enables translational studies; however, verify epitope conservation through sequence alignment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, providing end-to-end support from discovery to development. This BCAR1 polyclonal antibody, targeting a key mediator of cell adhesion and cancer signaling, complements a broad portfolio of validated antibodies, antibody pairs, and ancillary reagents. Whether you need bulk quantities or assistance with rare-target raw materials, CamelBio streamlines procurement to accelerate your diagnostic and research workflows.