Anti-BCAP29 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UHQ4

Core Product Specifications and Parameters

Biological Background: BCAP29 Function and Localization

• BCAP29, also known as BAP29 or B-cell receptor-associated protein 29, is a 28 kDa endoplasmic reticulum (ER) membrane protein encoded by the BCAP29 gene in humans.
• It is predicted to contain coiled-coil domains and transmembrane helices, as indicated by keywords including Coiled coil, Transmembrane, and Transmembrane helix.
• Functional studies suggest BCAP29 may facilitate anterograde transport of membrane proteins from the ER to the Golgi, playing a role in the early secretory pathway.
• By similarity, BCAP29 is potentially involved in CASP8-mediated apoptosis, linking ER functions to programmed cell death.
• The protein localizes specifically to the endoplasmic reticulum membrane, consistent with its transport and apoptosis-related roles.
• Alternative splicing of BCAP29 generates multiple isoforms, although the functional significance of these variants is not fully characterized.
• No tissue‑specific expression data are available, but proteomics identification confirms its presence in human reference proteomes.
• The UniProt record for BCAP29 (Q9UHQ4) includes the keyword 3D-structure, indicating that structural data may be available for this protein.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 125–241 of human BCAP29, a region that may be conserved across species, supporting the reported cross‑reactivity with mouse and rat.
• For Western blot (WB), a band at approximately 28 kDa is expected; consider optimizing sample preparation (e.g., reducing conditions) and antibody dilution based on endogenous expression levels in your model.
• In immunohistochemistry (IHC-P), validate staining specificity using tissue known to express BCAP29 (e.g., tissues with active secretory pathways) and include appropriate negative controls.
• For ELISA, ensure the recombinant immunogen or a closely matched target is used as coating antigen; perform checkerboard titrations to determine optimal working concentrations.
• Given the polyclonal nature of the antibody, lot‑to‑lot variability may occur; it is advisable to validate each new lot in the target application and sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, from validated antibody pairs to optimized monoclonal and polyclonal antibodies like this BCAP29 reagent. For studies on ER–Golgi trafficking and apoptosis, CamelBio can also source ancillary reagents and rare‑target antibodies, ensuring seamless progression from concept to clinic. With one‑stop access to bulk raw materials and technical consulting, CamelBio helps streamline your entire IVD development workflow.