Anti-BC12 Polyclonal Antibody for WB and ELISA - Q6YUL8

Core Product Specifications and Parameters

Biological Background: BC12 (Kinesin-like Protein KIN-4A) Function and Localization

• BC12, also known as Kinesin-like protein KIN-4A, is a microtubule-dependent motor protein that controls the oriented deposition of cellulose microfibrils, essential for cell wall biogenesis and modification. Related references: PMID:20444225
• BC12 acts as a transcriptional activator in the gibberellic acid (GA) biosynthesis pathway, binding the promoter of ent-kaurene oxidase 2 (CYP701A6) to regulate its expression, thereby mediating cell elongation. Related references: PMID:21325138
• BC12 contributes to cell-cycle progression and cell division. Related references: PMID:20444225
• BC12 localizes to the nucleus, cytoplasm, and cytoskeleton, consistent with its dual roles in transcriptional regulation and microtubule-based movement.
• BC12 is expressed in young tissues with active cell division, including root tips, flower primordia, intercalary meristems, and sub-epidermal regions of young culms and panicles.
• The protein contains an ATP-binding site, coiled-coil domains, and a DNA-binding motif, classifying it as a motor protein involved in growth regulation and transcription.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 840–970, a region predicted to be specific to Oryza sativa BC12; consider verifying sequence conservation if cross-reactivity with other species is desired.
• Western blot and ELISA are listed as suitable applications; it is recommended to optimize conditions and validate in the relevant sample system (e.g., rice tissue lysates).
• Due to its high molecular weight (~116 kDa), ensure appropriate gel percentage and transfer conditions for Western blot detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to streamline IVD raw-material procurement, offering validated antibodies, optimized reagent systems, and sourcing support for targets like BC12 in plant biology. Our portfolio covers cell wall and hormone research areas with supply-ready polyclonal antibodies and ancillary reagents to accelerate your pipeline.