Anti-BBS2 Rabbit Polyclonal Antibody for WB and ELISA - Q9BXC9

Core Product Specifications and Parameters

Biological Background: BBS2 Function and Localization

• BBS2 is a core component of the BBSome complex, a coat-like assembly essential for sorting membrane proteins to the primary cilium.
• The BBSome complex mediates ciliogenesis, in part through recruitment of the Rab8 guanine nucleotide exchange factor RAB3IP/Rabin8, leading to localized activation of Rab8-GTP and subsequent ciliary membrane extension.
• Together with the LZTFL1 protein, the BBSome controls the ciliary trafficking of Smoothened (SMO), thereby regulating the sonic hedgehog (SHH) signaling pathway.
• BBS2 is required for proper BBSome complex assembly and its localization to the cilium, underscoring its structural and functional role in complex integrity.
• Subcellularly, BBS2 localizes to the cilium membrane, cytoplasm, centrosome, and centriolar satellites, consistent with its role in trafficking at the primary cilium.
• The protein is widely expressed across tissues, reflecting the ubiquitous presence of primary cilia.
• Mutations in BBS2 cause Bardet-Biedl syndrome, a ciliopathy characterized by obesity, retinitis pigmentosa, intellectual disability, and other features, highlighting its importance in sensory transduction and transport.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 96 amino acids of human BBS2 (NP_114091.3); expect the antibody to recognize the full-length protein (~80 kDa) and potentially N-terminal fragments.
• For Western blot, consider using a positive control lysate from cells expressing BBS2 endogenously (e.g., HEK293) or from mouse/rat tissues, given the high sequence conservation in this region.
• The predicted molecular weight from UniProt is ~80 kDa (calculated mass 79.8 kDa), but observed migration may vary due to post-translational modifications or isoform differences; validate with appropriate molecular weight markers.
• ELISA application is listed; users should optimize coating antigen concentrations and antibody dilutions for their specific assay format.
• Cross-reactivity with mouse and rat suggests sequence homology; for species-specific applications, verify against lysates from non-reactive species as negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, providing diagnostic developers and researchers with bulk quantities of validated antibodies, recombinant proteins, and ancillary reagents. This BBS2 rabbit polyclonal antibody is ideal for studies of ciliopathies, Bardet-Biedl syndrome, and primary cilium biology. Contact our team to inquire about customized antibody formats, antibody pairs, and supply solutions tailored to your diagnostic or research needs.