Anti-BBS10 Rabbit Polyclonal Antibody for WB, ELISA - Q8TAM1

Core Product Specifications and Parameters

Biological Background: BBS10 Function and Localization

• BBS10 is a probable molecular chaperone that assists protein folding upon ATP hydrolysis, belonging to the chaperone family. Related references: PMID:20080638
• It plays a critical role in the assembly of the BBSome complex, which regulates transport vesicles to the primary cilium and is essential for ciliogenesis. Related references: PMID:20080638
• BBS10 is involved in adipogenic differentiation, as supported by functional studies. Related references: PMID:19190184
• Mutations in BBS10 cause Bardet-Biedl syndrome, a ciliopathy characterized by obesity, intellectual disability, retinal degeneration, and other sensory transduction defects.
• The protein localizes to the cell projection, specifically the cilium, in line with its role in ciliary trafficking.
• BBS10 is also known as C12orf58 (chromosome 12 open reading frame 58).

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 474–723 of human BBS10, targeting the C-terminal region. Consider confirming epitope specificity in your assays.
• For Western blot (WB), use whole-cell lysates from human or mouse cells expressing BBS10; a band at approximately 81 kDa is expected. Include appropriate controls (e.g., siRNA-mediated knockdown or knockout lysates) to verify antibody specificity.
• For ELISA, the antibody can be employed as a detection or capture reagent. Optimize coating antigen concentration and antibody dilution for the best signal-to-noise ratio.
• Given its cross-reactivity with human and mouse BBS10, this antibody is suitable for studies in both species, but validation in the specific experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For BBS10 and related ciliopathy targets, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and biomarker research.