Anti-BBOX1 Polyclonal Antibody for WB, IF-P, ELISA - O75936

Core Product Specifications and Parameters

Biological Background: BBOX1 Function and Localization

• BBOX1 encodes gamma-butyrobetaine dioxygenase (also known as gamma-butyrobetaine hydroxylase, gamma-BBH), a key enzyme in carnitine biosynthesis.
• It catalyzes the final step of L-carnitine synthesis: the hydroxylation of gamma-butyrobetaine to L-carnitine.
• The enzyme is a member of the non-heme Fe(II)/2-oxoglutarate-dependent dioxygenase family and requires iron and zinc as cofactors.
• BBOX1 is localized predominantly in the cytoplasm.
• Tissue expression is highest in the kidney, with moderate levels in the liver and very low levels in the brain.
• Post-translational modification includes phosphorylation (as indicated by the Phosphoprotein keyword).
• Carnitine is essential for fatty acid transport into mitochondria, so BBOX1 plays a crucial role in energy metabolism.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 1-180 of human BBOX1 (NP_003977.1). Consider that this N-terminal region may be conserved across species, supporting the reported cross-reactivity with mouse and rat.
• For Western blot (WB), a band around 45 kDa is expected (theoretical molecular weight ~45 kDa from UniProt). Validate with appropriate positive controls (e.g., kidney or liver lysates).
• For immunofluorescence (IF-P), anticipate cytoplasmic staining. Use appropriate fixation and permeabilization protocols.
• ELISA performance may be tested using recombinant BBOX1 protein or peptide antigens. Note that the antibody is a polyclonal, so consider potential batch-to-batch variability.
• Since BBOX1 expression is tissue-specific, selection of positive and negative control samples is critical for antibody validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research labs, providing high-quality raw materials from concept to clinic. In the area of metabolic enzyme research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support carnitine biosynthesis studies. For BBOX1 and related targets, our team can assist with rare-target raw-material sourcing to accelerate your assay development.