Anti-BAZ2A / TIP5 Rabbit Polyclonal Antibody for WB and ELISA - Q9UIF9

Core Product Specifications and Parameters

Biological Background: BAZ2A / TIP5 Function and Localization

• BAZ2A, also known as TIP5 or WALp3, is a large multidomain protein that serves as the regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes.
• These complexes catalyze ordered nucleosome spacing and facilitate DNA access during replication, transcription, and repair, with BAZ2A directly stimulating the ATPase activity of SMARCA5 in the NoRC-5 complex (PubMed:28801535). Related references: PMID:28801535
• Within the nucleolus, BAZ2A mediates rDNA silencing by recruiting histone deacetylases (e.g., HDAC1) and DNA methyltransferases to establish repressive heterochromatin, thereby inhibiting RNA polymerase I transcription.
• Targeting to rDNA is achieved through interaction with the transcription termination factor TTF1 and specific recognition of histone H4 acetylated at lysine 16 (H4K16ac), which subsequently triggers deacetylation of adjacent H4 residues.
• BAZ2A also binds promoter-associated RNAs (pRNAs) complementary to the rDNA promoter, a step essential for heterochromatin formation and transcriptional silencing.
• The protein localizes predominantly to the nucleolus, consistent with its role in ribosomal DNA regulation.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (via isopeptide bonds), as indicated by keywords in UniProt.
• Moderate expression is detected in many tissues, including heart, brain, placenta, lung, skeletal muscle, kidney, and pancreas.

Experimental Guidance and Technical Tips

• The immunogen was designed against a central region (aa 97-295) of human BAZ2A, which may provide good reactivity in Western blot and ELISA; consider validating in the relevant sample system.
• For Western blot, note that the predicted molecular weight is 211 kDa; ensure adequate protein transfer conditions and use appropriate molecular weight markers.
• Given the nuclear and nucleolar localization, appropriate subcellular fractionation may enhance detection in Western blot.
• The antibody cross-reacts with human and mouse; validate in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing gateway for high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. For targets like BAZ2A/TIP5 involved in chromatin remodeling and gene silencing, we can support procurement of custom polyclonal and monoclonal antibodies, bulk reagents, and rare target raw materials. Our integrated supply chain helps accelerate your assay development from concept to clinic.