Anti-BAZ2A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UIF9

Core Product Specifications and Parameters

Biological Background: BAZ2A Function and Localization

• BAZ2A, also known as TIP5 or hWALp3, is a regulatory subunit of the NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes that form ordered nucleosome arrays and facilitate DNA access during replication, transcription, and repair. Related references: PMID:28801535
• It directly stimulates the ATPase activity of SMARCA5 and regulates nucleosome spacing, with ATP hydrolysis rates differing between NoRC-1 and NoRC-5 complexes. Related references: PMID:28801535
• In the NoRC-5 complex, BAZ2A mediates rDNA silencing by recruiting histone-modifying enzymes such as HDAC1 and DNA methyltransferases, leading to heterochromatin formation and transcriptional repression of RNA polymerase I.
• It is recruited to rDNA via interaction with TTF1 and recognition of acetylated histone H4K16ac, resulting in deacetylation of other H4 lysines (K5, K8, K12).
• BAZ2A specifically binds pRNAs (150-250 nt) complementary to the rDNA promoter, a step required for heterochromatin formation and gene silencing.
• Subcellular localization is predominantly nucleolar.
• Tissue expression is moderate and widespread, including heart, brain, placenta, lung, skeletal muscle, kidney, and pancreas.
• The protein features bromodomains, zinc-fingers, coiled-coil regions, and undergoes post-translational modifications such as acetylation, phosphorylation, and ubiquitylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to aa 97-295 of human BAZ2A; cross-reactivity with mouse and rat suggests sequence conservation, supporting use in these species.
• For Western blot, expect a band at approximately 211 kDa; consider extended gel electrophoresis and transfer conditions for large proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate antibody specificity with appropriate controls and optimize antigen retrieval.
• This polyclonal antibody can be used in ELISA; for quantitative assays, confirm linearity and sensitivity in the relevant matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents. This anti-BAZ2A antibody supports chromatin remodeling and rDNA silencing research, and CamelBio can source complementary raw materials for your entire workflow. Simplify your supply chain with our integrated procurement services, spanning concept to clinic.