Anti-Bax Rabbit Polyclonal Antibody (KO Validated) for Apoptosis Research - Q07812

Core Product Specifications and Parameters

Biological Background: Bax Function and Localization

• Bax is a central apoptosis regulator that drives mitochondrial outer membrane permeabilization. Upon cellular stress, it undergoes a conformational change, translocates to the mitochondrial outer membrane, and triggers cytochrome c release, which activates caspase-3 and downstream apoptosis. Related references: PMID:10772918 PMID:11060313 PMID:16113678
• Under normal conditions, Bax is maintained in an inactive state largely in the cytosol through constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, preventing toxic mitochondrial accumulation. Related references: PMID:21458670
• Bax is expressed in a wide variety of tissues. Multiple isoforms are generated by alternative splicing: isoform Psi is found in glial tumors; isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, with low levels in skin and lung; isoform Sigma shows a similar tissue distribution and is also present in many cancer cell lines.
• Subcellularly, Bax localizes to the mitochondrion outer membrane, cytoplasm, and nucleus, with its distribution shifting in response to apoptotic signals.
• Post-translational modifications such as acetylation, Ubl conjugation, and isopeptide bond formation may modulate Bax activity and stability.
• Bax is classified as a tumor suppressor and has been implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human Bax, which is highly conserved across human, mouse, and rat, likely contributing to the observed cross-reactivity. When validating in your model system, confirm specificity via knockdown or knockout controls.
• For western blot (WB), expect a band at ~21 kDa. Use reducing conditions and appropriate loading controls; consider that multiple isoforms may produce additional bands.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and fixation methods can influence detection of cytosolic versus mitochondrial Bax pools. Pilot experiments are advised to optimize conditions.
• This polyclonal antibody may be suitable for immunoprecipitation (IP) and ELISA. For ELISA, the peptide immunogen can serve as a standard for quantitation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for apoptosis and cancer research. For advanced Bax studies or related apoptosis markers, our team can assist with tailored sourcing and technical support.