Anti-Bax Rabbit Monoclonal Antibody (KO Validated) for WB, IF/ICC, IP, ELISA - Q07812

Core Product Specifications and Parameters

Biological Background: Bax Function and Localization

• Bax (BCL2L4) is a pro-apoptotic member of the Bcl-2 family that plays a pivotal role in the mitochondrial apoptotic process.
  Related references: PMID:10772918, PMID:11060313, PMID:16113678
• Under normal conditions, Bax is predominantly cytosolic due to continuous retrotranslocation from the mitochondrial outer membrane, a process mediated by BCL2L1/Bcl-xL to prevent toxic accumulation.
  Related references: PMID:21458670
• In response to stress signals, Bax undergoes a conformational change, translocates to the mitochondria, and promotes the release of cytochrome c, which activates caspase-3 and triggers apoptosis.
  Related references: PMID:10772918, PMID:11060313, PMID:16113678
• Bax is widely expressed in human tissues, with specific isoforms (Psi, Alpha, Sigma) differentially found in various cancers and normal tissues, including glial tumors, spleen, breast, ovary, and lung.
• Its subcellular localizations include the mitochondrial outer membrane, cytoplasm, and nucleus, and it is subject to post-translational modifications such as acetylation, isopeptide bond formation, and ubiquitination.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to a conserved N-terminal region (amino acids 10-70) of human Bax, this antibody is expected to detect Bax in human, mouse, and rat samples. However, validation in your specific sample system is recommended.
• For Western blotting, a band at approximately 21 kDa is expected based on the predicted molecular weight. Using reduced or non-reduced conditions may be considered to assess potential isoforms.
• Suitable applications include WB, IF/ICC, IP, and ELISA. Optimal dilutions and conditions should be empirically determined.
• Include appropriate positive and negative controls (e.g., apoptotic vs. non-apoptotic cell lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-Bax monoclonal antibody is ideal for apoptosis research and can be sourced along with complementary raw materials for your assay development needs. Our team also supports rare target raw-material sourcing and technical consulting to streamline your project from concept to clinic.