Anti-Bax Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q07812

Core Product Specifications and Parameters

Biological Background: Bax Function and Localization

• BAX is an apoptosis regulator and a member of the Bcl-2 family; it is also known as Bcl-2-like protein 4 (Bcl2-L-4).
• Under normal conditions, BAX is primarily cytosolic and is continuously retrotranslocated from mitochondria to the cytosol by BCL2L1/Bcl-xL, preventing toxic accumulation at the mitochondrial outer membrane. Related references: PMID:21458670
• Upon apoptotic stimuli, BAX undergoes a conformational change, translocates to the mitochondrial outer membrane, and promotes the release of cytochrome c, which triggers the intrinsic apoptosis pathway. Related references: PMID:10772918, PMID:11060313, PMID:16113678
• BAX promotes the activation of caspase-3 (CASP3), thereby executing apoptosis. Related references: PMID:10772918, PMID:11060313, PMID:16113678
• BAX localizes to multiple cellular compartments including the cytoplasm, mitochondrial outer membrane, and nucleus, reflecting its dynamic role in apoptosis regulation.
• BAX is widely expressed in human tissues, with specific isoforms (Alpha, Sigma, Psi) detected in various cancers such as leukemia, lymphoma, breast, ovary, prostate, and lung carcinomas.
• Key post-translational modifications include acetylation and ubiquitination, and alternative splicing produces multiple isoforms with distinct expression patterns.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human, mouse, or rat cell lines; a band of approximately 21 kDa is expected for the full-length BAX protein. Inclusion of positive controls such as staurosporine-treated cells may help confirm specificity.
• In IHC-P, antigen retrieval with citrate buffer (pH 6.0) may enhance detection; validate using tissues with known expression (e.g., spleen, breast, colon).
• For IF/ICC, confirm subcellular redistribution of BAX under apoptotic conditions, with appropriate fixation and permeabilization protocols.
• In ELISA, pairing with a validated BAX capture antibody could enable sandwich assay development; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, from concept to clinic. For apoptosis research and assay development, we offer validated anti-BAX antibody pairs, optimized polyclonal antibodies, and ancillary reagents, including custom sourcing for rare targets. Partner with us to streamline your raw-material procurement and accelerate project timelines.