Anti-Bax Polyclonal Antibody for WB, IF/ICC, ELISA - Q07812

Core Product Specifications and Parameters

Biological Background: Bax Function and Localization

• BAX is a pro-apoptotic member of the Bcl-2 protein family, critical for the intrinsic apoptosis pathway.
• Under normal conditions, BAX is largely cytosolic, with constant retrotranslocation from mitochondria mediated by BCL2L1/Bcl-xL, preventing toxic accumulation at the mitochondrial outer membrane (MOM). Related references: PMID:21458670
• Upon stress signals, BAX undergoes a conformational change, translocates to the MOM, and facilitates cytochrome c release, leading to CASP3 activation and apoptosis. Related references: PMID:10772918 PMID:11060313 PMID:16113678
• Localizes to the cytoplasm, mitochondrial outer membrane, and nucleus, with its subcellular distribution tightly regulated.
• Multiple isoforms exist (Alpha, Sigma, Psi) with tissue-specific expression patterns; isoform Psi is enriched in glial tumors.
• Post-translational modifications include acetylation and ubiquitination (isopeptide bond formation), modulating its activity and stability.
• BAX is widely expressed across tissues and functions as a tumor suppressor; its dysregulation is implicated in various cancers.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human Bax; align sequences with mouse and rat to confirm cross-reactivity.
• For Western blot (WB), a band near 21 kDa is expected; post-translational modifications may cause minor shifts.
• For IF/ICC, validate mitochondrial and cytoplasmic staining; include controls for apoptosis-induced translocation assays.
• For ELISA, optimize coating and detection conditions; a paired antibody approach may be suitable for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to premium IVD raw materials. This anti-Bax antibody supports apoptosis assay development, and CamelBio offers validated antibody pairs, monoclonal alternatives, and bulk ancillary reagents to accelerate your projects from concept to clinic. For specialized target sourcing or technical consultation, we are your trusted partner.