Anti-BAT3/BAG6 Rabbit Monoclonal Antibody for WB and ELISA - P46379

Core Product Specifications and Parameters

Biological Background: BAT3/BAG6 Function and Localization

• BAT3/BAG6 (BAG family molecular chaperone regulator 6), also known as HLA-B-associated transcript 3, Protein G3, and Protein Scythe, is a large proline-rich protein encoded by the BAG6 gene in humans. It functions as an ATP-independent molecular chaperone that prevents aggregation of misfolded and hydrophobic patch-containing proteins, operating as part of a cytosolic protein quality control complex. Related references: PMID:21636303

• BAG6 plays a central role in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum (ER) membrane, where it is recruited to ribosomes, interacts with the transmembrane region of nascent proteins, and cooperates with SGTA and ASNA1. Mislocalized or improperly delivered client proteins are ubiquitinated by RNF126 and directed to the proteasome for degradation, a process negatively regulated by SGTA. Related references: PMID:20516149, PMID:20676083, PMID:25535373, PMID:24981174, PMID:27193484, PMID:28104892, PMID:23129660, PMID:25179605

• In the context of ER-associated degradation (ERAD), BAG6 maintains retrotranslocated proteins in a soluble state in the cytosol, ensuring their proper targeting to the proteasome. It is also implicated in the degradation of defective nascent chain polypeptides, potentially contributing to antigen presentation and immune responses. Related references: PMID:21636303

• Beyond protein quality control, BAG6 is involved in DNA damage-induced apoptosis: upon DNA damage, it accumulates in the nucleus, forms a complex with p300/EP300 to enhance p53 acetylation, and increases p53 transcriptional activity. It may also regulate histone H3K4 dimethylation as part of a chromatin regulator complex. Related references: PMID:17403783, PMID:18765639

• BAG6 positively regulates apoptosis by stabilizing the proapoptotic factor AIFM1 and mediates ricin-induced apoptosis. Additionally, it indirectly controls the insulin-like growth factor receptor signaling pathway by regulating IGF1R steady-state expression. Related references: PMID:26692333

• Extracellularly, BAG6 is released via exosomes and serves as a ligand for the natural killer cell receptor NCR3, stimulating NK cell cytotoxicity against tumor cells and immature dendritic cells.

• Subcellular localization includes the cytosol, nucleus, and extracellular exosomes. It is expressed by immature dendritic cells at the protein level and is subject to acetylation, phosphorylation, and ubiquitination, reflecting its dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 1033-1132) of human BAG6, suggesting the antibody recognizes an epitope in this region. When designing experiments, consider that this region may be present in all known isoforms, but confirm isoform-specific detection if required.

• The antibody is validated for WB and ELISA. For Western blot, the predicted molecular weight is 119 kDa. Ensure adequate separation on SDS-PAGE with appropriate molecular weight markers and optimize transfer conditions for large proteins.

• Given the reported cross-reactivity with human, mouse, and rat, the antibody may be suitable for studies across these species. Validate in the relevant sample system, taking into account potential differences in expression levels or post-translational modifications.

• BAG6 is involved in multiple cellular processes; consider using appropriate positive and negative controls (e.g., cell lines with known BAG6 expression, siRNA knockdown) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This BAT3/BAG6 antibody supports investigations into protein quality control, apoptosis, and immune surveillance—key areas for biomarker discovery and assay development. Whether you require routine catalog products or custom sourcing for rare targets, CamelBio bridges the gap from concept to clinic with reliable supply and technical consultation.