Anti-BAT3/BAG6 Polyclonal Antibody for WB, ELISA - P46379

Core Product Specifications and Parameters

Biological Background: BAT3/BAG6 Function and Localization

• Protein identity and gene family: Large proline-rich protein BAG6, also known as BAG family molecular chaperone regulator 6 (BAG-6), HLA-B-associated transcript 3 (BAT3), Protein G3, and Scythe. Encoded by the BAG6 gene (GeneID: 7917) and belonging to the BAG family of co-chaperones.
• Chaperone-mediated protein quality control: Functions as an ATP-independent molecular chaperone that prevents aggregation of misfolded and hydrophobic proteins, acting as part of the BAG6/BAT3 complex to maintain client proteins soluble for delivery to the endoplasmic reticulum or targeting to the proteasome. Related references: PMID:21636303, PMID:20516149, PMID:21743475
• Tail-anchored protein delivery: Recruited to ribosomes, interacts with the transmembrane region of newly synthesized tail-anchored proteins, and together with SGTA and ASNA1 mediates their delivery to the ER. Related references: PMID:20516149, PMID:20676083, PMID:25535373
• Ubiquitin-dependent sorting and degradation: Client proteins that fail ER delivery are ubiquitinated by RNF126 and sorted to the proteasome; SGTA negatively regulates this process by preventing RNF126 recruitment. Related references: PMID:24981174, PMID:27193484, PMID:28104892, PMID:23129660, PMID:25179605
• Endoplasmic reticulum stress response: Involved in pre-emptive quality control during ER stress, rerouting nascent proteins to the cytosol for proteasomal degradation to protect the ER from overload. Related references: PMID:26565908
• DNA damage and chromatin regulation: In response to DNA damage, accumulates in the nucleus, forms a complex with p300/EP300 to enhance p53 acetylation and transcriptional activity. May also regulate H3K4 dimethylation as part of a chromatin regulator complex. Related references: PMID:17403783, PMID:18765639
• Immune function and secretion: Released extracellularly via exosomes, acts as a ligand for NK cell receptor NCR3, stimulating NK cell cytotoxicity against tumor cells and immature dendritic cells.
• Subcellular localization and tissue specificity: Distributed in the cytosol, nucleus, and secreted via exosomes. Expressed at protein level in immature dendritic cells. Key post‑translational modifications include acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• Immunogen Design: The immunogen corresponds to the N‑terminal region (amino acids 1‑200) of human BAT3/BAG6. This region may be conserved across species, consistent with observed cross‑reactivity with mouse and rat.
• Western Blotting (WB): Consider using whole‑cell lysates from human, mouse, or rat cell lines as positive controls. The predicted molecular weight is ~119 kDa; validate band specificity with appropriate blocking and detection conditions.
• ELISA Applications: For sandwich ELISA development, consider pairing with a matched monoclonal antibody targeting a distinct epitope. Validate assay performance with recombinant protein standards.
• General Note: Always validate antibody performance in your specific experimental system, as optimal dilutions and conditions may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one‑stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like the BAT3/BAG6 Rabbit pAb, and bulk ancillary reagents. We support researchers and diagnostic developers studying molecular chaperone pathways, protein quality control, apoptosis, and immune responses by providing reliable access to critical reagents and rare target raw material sourcing. Contact our team to streamline your procurement and accelerate development timelines.