Anti-BAP31 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P51572

Core Product Specifications and Parameters

Biological Background: BAP31 Function and Localization

• BAP31 (BCAP31) is a 28 kDa endoplasmic reticulum (ER) membrane protein, also known as 6C6-AG tumor-associated antigen and protein CDM/p28.
• Functions as a chaperone and is one of the most abundant ER proteins, playing a key role in the export of secreted proteins and the recognition/targeting of misfolded proteins for ER-associated degradation (ERAD). Related references: PMID:18287538 PMID:9396746
• Serves as a cargo receptor for the export of transmembrane proteins (By similarity).
• Plays a role in mitochondrial Complex I assembly by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40. Related references: PMID:31206022
• Upon ER stress, BAP31 delocalizes from ER-mitochondria contact sites and binds BCL2; may be involved in CASP8-mediated apoptosis. Related references: PMID:31206022 PMID:10958671
• Subcellularly, BAP31 localizes to the endoplasmic reticulum membrane and the ER-Golgi intermediate compartment membrane.
• Ubiquitously expressed, with high expression in neurons and discrete endocrine cells.
• Genetic variants in BCAP31 are linked to disorders such as deafness, dystonia, and intellectual disability.
• Alternative splicing generates multiple isoforms, and the protein features a coiled-coil domain and transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 127–246 of human BAP31; confirm epitope accessibility in your assay format.
• For western blot, a specific band near 28 kDa is expected; validate detection conditions using positive control lysates from human, mouse, or rat cells.
• For immunofluorescence/ICC, consider fixation and permeabilization protocols suitable for ER membrane proteins.
• The antibody is suitable for immunoprecipitation and ELISA applications; optimize concentrations and buffer conditions for each assay.
• Cross-reactivity with mouse and rat BAP31 is reported; verify performance in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials, from validated antibody pairs to bulk ancillary reagents. The BAP31 antibody strengthens our portfolio of ER and protein trafficking research tools, supporting your assay development and biomarker discovery workflows. Whether you need optimized monoclonal/polyclonal antibodies or rare target sourcing, CamelBio bridges the gap from concept to clinic with reliable, scalable solutions.