Anti-BAP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q92560

Core Product Specifications and Parameters

Biological Background: BAP1 Function and Localization

• BAP1 is a ubiquitin carboxyl-terminal hydrolase that functions as a deubiquitinating enzyme, removing ubiquitin from histone H2A and HCFC1, thereby regulating chromatin dynamics and gene expression. Related references: PMID:12485996, PMID:18757409, PMID:20436459
• As the catalytic subunit of the Polycomb repressive deubiquitinase (PR-DUB) complex, it specifically mediates deubiquitination of histone H2A monoubiquitinated at Lys-120 (H2AK119ub1), but not H2B. Related references: PMID:20436459, PMID:25451922, PMID:30664650
• The PR-DUB complex acts as a transcriptional coactivator by antagonizing PRC1-mediated H2AK119ub1 monoubiquitination and is recruited to gene-regulatory regions by YY1, influencing genes involved in development, cell proliferation, and viability. Related references: PMID:20805357, PMID:30664650, PMID:36180891
• BAP1 regulates cell growth by deubiquitinating HCFC1, showing preference toward Lys-48-linked over Lys-63-linked polyubiquitin chains, though this does not affect HCFC1 stability. Related references: PMID:19188440, PMID:19815555
• It negatively regulates epithelial-mesenchymal transition (EMT) in trophoblast stem cells during placental development, maintaining epithelial integrity and cell adhesion. Related references: PMID:34170818
• BAP1 undergoes autodeubiquitination to protect its nuclear localization signal from monoubiquitination, preventing cytoplasmic sequestration; it is also a phosphoprotein. Related references: PMID:24703950
• It is highly expressed in testis, placenta, ovary, and breast tissue, with levels increasing during pregnancy, and localizes to the cytoplasm, nucleus, and chromosomes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-270 of human BAP1 (NP_004647.1), which includes the ubiquitin catalytic domain; this design may generate antibodies against multiple epitopes within this region.
• For WB, BAP1 has a predicted molecular weight of 80 kDa; validate with appropriate positive controls (e.g., human, mouse, or rat cell lysates).
• In IHC-P and IF/ICC, optimize antigen retrieval and antibody dilution; nuclear staining is expected.
• ELISA can be employed for antibody titer determination; consider using the recombinant immunogen as a coating antigen.
• Cross-reactivity with mouse and rat BAP1 is reported; verify conservation of the immunogen sequence in these species for accurate detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop sourcing of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and custom polyclonal/monoclonal antibody development. For chromatin and deubiquitination research, our portfolio supports the procurement of high-quality antibodies like this anti-BAP1 pAb, along with technical consultation from concept to clinical application.