Anti-BANP Polyclonal Antibody for WB, ELISA - Q8N9N5

Core Product Specifications and Parameters

Biological Background: BANP Function and Localization

• BANP (BEN domain-containing protein 1, SMAR1) is a nuclear protein that functions as a tumor suppressor and transcriptional repressor, controlling V(D)J recombination, cell cycle progression, and alternative splicing.
• It represses T-cell receptor (TCR) beta enhancer function by binding to scaffold/matrix attachment region beta (S/MARbeta), thereby regulating V(D)J recombination during T-cell development.
• BANP represses cyclin D1 transcription by recruiting HDAC1 to its promoter, leading to reduced histone acetylation and cell cycle arrest. Related references: PMID:16166625
• It promotes TP53 activation, causing cell cycle arrest, and plays a role in alternative splicing by binding to CD44 pre-mRNA and negatively regulating the inclusion of proximal variable exons v2-v6. Related references: PMID:26080397
• Subcellular localization includes nucleus, nuclear speckles, and cytoplasm, consistent with its functions in transcription regulation and RNA processing.
• Down-regulated in breast cancer cell lines, supporting its tumor suppressor role. Post-translational modifications include acetylation, phosphorylation, and ubiquitination.
• Contains a BEN domain and is categorized under keywords including chromatin regulator, DNA-binding, RNA-binding, and developmental protein.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-260) which includes the BEN domain; this region is conserved, so consider testing cross-reactivity with other species.
• For Western blotting, use nuclear extracts from human or mouse T-cell lines (e.g., Jurkat) to enrich BANP signal, given its nuclear localization.
• Validate antibody specificity by comparing lysates from BANP-overexpressing versus knockdown cells, or by using control tissues with known expression levels.
• ELISA applications may benefit from coating with the recombinant immunogen fragment to optimize detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw material procurement, supplying validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our BANP antibody aids researchers studying T-cell development, chromatin regulation, and tumor suppression. We also excel in sourcing rare-target raw materials, ensuring a seamless and reliable supply chain from concept to clinical application.