Anti-Bak Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q16611

Core Product Specifications and Parameters

Biological Background: Bak Function and Localization

• The BAK protein, also known as Bcl-2 homologous antagonist/killer, apoptosis regulator BAK, or Bcl2-L-7, is encoded by the BAK1 gene (aliases: BAK, BCL2L7, CDN1).
• BAK plays a critical role in the intrinsic apoptotic pathway by promoting mitochondrial outer membrane (MOM) permeabilization upon cell death signals, leading to cytochrome c release and caspase cascade activation.
• It belongs to the Bcl-2 family and functions as a pro-apoptotic effector, directly oligomerizing to form pores in the MOM.
• BAK is localized to the mitochondrial outer membrane, consistent with its apoptotic function at this organelle.
• It is widely expressed across tissues, with notably high levels in the heart and skeletal muscle.
• Post-translational modifications include acetylation and zinc binding; the protein contains transmembrane helices and is subject to alternative splicing.
• The protein is also involved in host-virus interactions, reflecting its regulatory role beyond core apoptosis.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa 50–150) targets an N-terminal region, which may detect both full-length and potential processed forms of BAK.
• For Western blot, expect a band around 23 kDa; use appropriate loading controls and validate detection in your sample system.
• In IF/ICC, anticipate mitochondrial staining; co-localization with mitochondrial markers is recommended.
• For IP, optimize antibody concentration and lysis buffer conditions to preserve protein complexes.
• Given cross-reactivity with mouse and rat, this antibody is suitable for multi-species apoptosis studies after appropriate validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research groups with streamlined access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-Bak pAb, and ancillary reagents. We help you source rare targets and scale up procurement, simplifying your path from apoptosis research to clinical assay development.