Anti-Bak Rabbit Polyclonal Antibody for WB, ELISA - Q16611

Core Product Specifications and Parameters

Biological Background: Bak Function and Localization

• Bak (Bcl-2 homologous antagonist/killer), also known as apoptosis regulator BAK or Bcl2-L-7, is a pro-apoptotic member of the Bcl-2 protein family.
• Encoded by the BAK1 gene (Gene ID: 578), it plays a critical role in the intrinsic mitochondrial apoptotic pathway.
• Upon apoptotic stimuli, Bak oligomerizes and forms pores in the mitochondrial outer membrane (MOM), leading to permeabilization and release of apoptogenic factors such as cytochrome c into the cytosol.
• This release triggers the activation of caspase 9 and downstream effector caspases, executing the cell death program.
• Bak is primarily localized to the mitochondrial outer membrane, consistent with its function in apoptosis regulation.
• It is widely expressed across human tissues, with the highest expression levels in the heart and skeletal muscle.
• Post-translational modifications, including acetylation and zinc binding, may regulate Bak activity, and alternative splicing generates multiple isoforms.
• The protein interacts with host factors during viral infection, highlighting its role in host-virus interactions.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to the N-terminal 1–100 amino acids of human Bak; it is expected to recognize full-length endogenous Bak protein.
• For Western blotting, the predicted molecular weight is ~23 kDa; consider using mitochondrial-enriched fractions or positive control lysates from heart or skeletal muscle where Bak expression is highest.
• When performing ELISA, titrate the antibody to determine optimal coating and detection concentrations, and validate specificity using recombinant Bak protein or peptide competition assays.
• Cross-reactivity with mouse is reported; however, validate in your specific sample system, as detection may vary with sample preparation and epitope accessibility.
• Since Bak is a mitochondrial membrane protein, ensure proper lysis buffers and denaturing conditions to expose the epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers convenient access to high-quality IVD raw materials like this anti-Bak antibody, supporting apoptosis and cancer research. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents in bulk, along with rare-target raw-material sourcing to accelerate your assay development from concept to clinic.