Anti-BAIAP2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UQB8

Core Product Specifications and Parameters

Biological Background: BAIAP2 Function and Localization

• BAIAP2 (BAI1-associated protein 2), also known as IRSp53, FLAF3, BAP2, and WAML, is a BAR/IMD domain-containing adapter protein. It exists in multiple isoforms due to alternative splicing; isoform 1 and isoform 4 are predominantly expressed in the brain, while a short isoform is ubiquitously expressed with highest levels in liver, prostate, testis, and placenta.
• Core Function: BAIAP2 links membrane-bound small G proteins (CDC42, RAC1) to cytoplasmic effector proteins, regulating actin cytoskeleton reorganization. It is required for CDC42-mediated actin reorganization and RAC1-driven membrane ruffling, and works with WASF family proteins and the Arp2/3 complex to remodel actin.
• Neuronal Roles: BAIAP2 promotes neurite growth, neuronal differentiation, and neuronal migration. It acts synergistically with ENAH to drive filopodia formation, which is critical for neuronal development and synaptic connectivity. Related references: PMID:41133935
• Actin Bundling and Filopodia: Upon association with EPS8, BAIAP2 induces actin bundling and filopodial protrusions, a process essential for cell migration and pathogen uptake during bacterial infection. Related references: PMID:41133935
• Subcellular Localization: BAIAP2 localizes to the cytoplasm, plasma membrane, and cytoskeleton, and is enriched at cell protrusions including filopodia and membrane ruffles, consistent with its actin regulatory function.
• Post-Translational Modification: BAIAP2 is a phosphoprotein; its activity and interactions are modulated by phosphorylation, which likely influences its role in signaling and cytoskeletal dynamics.
• Disease Relevance: Mutations in BAIAP2 are associated with neurological disorders, including epilepsy and intellectual disability, underscoring its importance in brain development and function.
• Tissue Distribution: While some isoforms are brain-specific, a shorter isoform is widely expressed across tissues, with notably high levels in liver, prostate, testis, and placenta, suggesting diverse functional roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 362-521) of human BAIAP2; this region may be conserved across species, enabling detection of human, mouse, and rat orthologs.
• For Western blotting, the expected molecular weight is approximately 61 kDa; however, multiple bands corresponding to splice isoforms or phospho-forms may appear. Titrate antibody concentration and validate in relevant tissue or cell lysates (e.g., brain extracts).
• In immunocytochemistry/immunofluorescence (IF/ICC), expect staining at filopodia, membrane ruffles, and cytoplasm. Fixation and permeabilization conditions should be optimized for your cell type.
• For ELISA, the antibody can be used as a capture or detection reagent; pair with a matched antibody or use individually for antigen detection, and validate with recombinant BAIAP2 protein or peptide.
• Cross-reactivity with mouse and rat is reported; however, sequence alignment of the immunogen region is recommended to ensure homology before use in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, providing high-quality antibodies for cytoskeletal and neuroscience research. In addition to this anti-BAIAP2 polyclonal antibody, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, CamelBio supports your diagnostic development with reliable supply-chain solutions.