Anti-BAG5 Polyclonal Antibody for WB, IHC-P, ELISA - Q9UL15

Core Product Specifications and Parameters

Biological Background: BAG5 Function and Localization

• BAG5, also known as Bcl-2-associated athanogene 5 or BAG-5, is a member of the BAG domain-containing protein family that functions as co-chaperones.
• It acts as a nucleotide-exchange factor for HSP70/HSPA proteins, accelerating ADP release and driving the chaperone cycle to promote protein refolding.

Related references: PMID:20223214

• At junctional membrane complexes (JMC), BAG5 is essential for proteostasis, likely by scaffolding between HSPA8 chaperone and JMC proteins to facilitate proper folding.
• BAG5 inhibits the E3 ubiquitin-protein ligase PRKN, suppressing both auto-ubiquitination of PRKN and ubiquitination of its substrate proteins.
• Primarily expressed in the heart, BAG5 is linked to cardiomyopathy; disease-associated variants have been identified.
• The protein undergoes alternative splicing and contains multiple BAG domains.
• The full-length protein has a molecular weight of approximately 51-52 kDa.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 198-447) targets the C-terminal portion of BAG5, including functional BAG domains; this design may detect full-length and certain splice variants.
• For Western blotting, the predicted band is ~51-52 kDa; verify in relevant tissue/cell lysates (e.g., heart samples) and consider using a positive control expressing BAG5.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and test appropriate antibody dilutions; cardiac muscle sections may serve as a positive control given heart expression.
• ELISA applications can use recombinant BAG5 protein or peptide fragments for standard curve generation and antibody titer determination.
• As a polyclonal antibody, lot-to-lot consistency should be evaluated; include appropriate negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio offers comprehensive sourcing solutions from discovery to commercialization. This anti-BAG5 antibody supports research into chaperone biology and cardiac proteostasis, and CamelBio can supply validated antibody pairs, bulk ancillary reagents, and custom raw-material procurement to streamline your diagnostic development pipeline.