Anti-BAG4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O95429

Core Product Specifications and Parameters

Biological Background: BAG4 Function and Localization

• BAG4, also known as Bcl-2-associated athanogene 4 or Silencer of death domains (SODD), is a member of the BAG family of molecular chaperone regulators (UniProt O95429).
• Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release, thereby preventing constitutive TNFRSF1A signaling (UniProt O95429).
• Negatively regulates PRKN translocation to damaged mitochondria, suggesting a role in mitochondrial quality control (UniProt O95429).
• Localizes predominantly to the cytoplasm (UniProt O95429).
• Expressed ubiquitously across tissues (UniProt O95429).
• Subject to post-translational modifications including methylation and phosphorylation; alternative splicing generates multiple isoforms (UniProt O95429).

Experimental Guidance and Technical Tips

• For Western blotting, a specific band around 50 kDa is expected; optimize protein loading and use appropriate positive controls (e.g., human, mouse, rat cell lysates). Confirm specificity with knockdown or knockout samples if possible.
• For immunohistochemistry on paraffin-embedded sections, antigen retrieval is critical; start with heat-induced epitope retrieval in citrate buffer (pH 6.0) and titrate antibody concentration to minimize background.
• In ELISA, this polyclonal antibody can serve as a capture or detection reagent; validate pairing with your recombinant antigen or native protein in a checkerboard titration to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with a comprehensive range of IVD raw materials and tailored technical support, accelerating development from concept to clinic. This anti-BAG4 polyclonal antibody is part of an extensive portfolio for apoptosis, chaperone biology, and cell signaling research. Beyond validated antibody pairs and optimized monoclonal/polyclonal antibodies, we offer bulk ancillary reagents and can source rare-target raw materials to meet your specific workflow needs.