Anti-BAG3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O95817

Core Product Specifications and Parameters

Biological Background: BAG3 Function and Localization

• Co-chaperone and adapter protein that connects heat shock proteins 70 (HSP70s) and small heat shock proteins (sHSPs), acting as a nucleotide-exchange factor (NEF) to promote client release from HSP70s. Related references: PMID:27884606, PMID:30559338, PMID:27474739, PMID:9873016
• Possesses anti-apoptotic activity, contributing to cellular survival under stress conditions. Related references: PMID:10597216
• Regulates the nucleocytoplasmic transport of HSF1, affecting heat shock response. Related references: PMID:26159920
• Localizes to both the nucleus and cytoplasm, consistent with its roles in chaperone machinery and stress signaling.
• Associated with diverse pathologies including cardiomyopathy, Charcot-Marie-Tooth disease, myofibrillar myopathy, and neurodegeneration, as highlighted by disease variant keywords.
• Undergoes multiple post-translational modifications such as phosphorylation, acetylation, methylation, and ubiquitination, which may modulate its function and stability.
• Belongs to the BAG family of co-chaperones, also known as Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, or docking protein CAIR-1.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 380–575), which is expected to detect full-length BAG3 in most species with high homology; consider verifying isoform specificity based on your experimental system.
• For Western blotting (WB), a predominant band at ~62 kDa is anticipated; include appropriate positive and negative controls to validate detection specificity.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution using tissues with known BAG3 expression, as localization may vary between normal and disease states.
• ELISA can be employed for titer determination or screening applications; use the recombinant protein as a standard and validate with a compatible detection system.
• As a polyclonal antibody, lot-to-lot variability is possible; always titrate and confirm performance with your specific samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with a comprehensive one-stop platform for IVD raw materials, technical services, and consulting. For your BAG3-related studies—spanning chaperone biology, apoptosis, and neurodegeneration—we supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and can source rare targets to accelerate your assay development from concept to clinic.