Anti-BAG1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99933

Core Product Specifications and Parameters

Biological Background: BAG1 Function and Localization

• Acts as a co-chaperone for HSP70 and HSC70, functioning as a nucleotide-exchange factor (NEF) to promote ADP release and client protein release (PubMed:24318877, PubMed:27474739, PubMed:9873016). Related references: PMID:24318877, PMID:27474739, PMID:9873016
• Inhibits the pro-apoptotic function of PPP1R15A, contributing to its anti-apoptotic activity (PubMed:12724406). Related references: PMID:12724406
• Enhances the anti-cell death function of BCL2 in response to various apoptotic stimuli (PubMed:9305631). Related references: PMID:9305631
• Localizes to both the cytoplasm and nucleus, consistent with its roles in chaperone regulation and apoptosis.
• Ubiquitously expressed (isoform 4) except in liver, colon, breast, and uterine myometrium; isoform 1 found in ovary and testis; expression is elevated in leukemia and lymphoma cell lines.
• Undergoes alternative initiation and splicing to generate multiple isoforms, and is regulated by phosphorylation and ubiquitin-like modifications.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-230 at the N-terminal region of human BAG1, which may recognize multiple isoforms.
• For western blotting, the predicted molecular weight is approximately 39 kDa for the full-length isoform; other isoforms may exhibit different mobilities.
• Verify antibody specificity in your target sample system using appropriate positive and negative controls.
• Optimize blocking conditions and primary antibody dilution for each specific application (WB, IF/ICC, ELISA).
• Consider using recombinant protein or cell lysates with known BAG1 expression as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive IVD raw material sourcing, from concept to clinic. This BAG1 polyclonal antibody is well-suited for studies on apoptosis, chaperone biology, and cancer biomarker development. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and access to difficult-to-source targets to streamline your assay development and production workflows.