Anti-BAG1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99933

Core Product Specifications and Parameters

Biological Background: BAG1 Function and Localization

• BAG1 encodes the BAG family molecular chaperone regulator 1, also known as Bcl-2-associated athanogene 1 (BAG-1). It is a member of the BAG co-chaperone family that interacts with HSP70/HSC70 chaperones.
• Acts as a nucleotide-exchange factor (NEF) for HSP70/HSC70, promoting the release of ADP and triggering client/substrate protein release via binding to both the nucleotide-binding domain (NBD) and substrate-binding domain (SBD) of HSPA8/HSC70. Related references: PMID:24318877 PMID:27474739 PMID:9873016
• Exhibits anti-apoptotic activity by inhibiting the pro-apoptotic function of PPP1R15A and markedly increasing the anti-cell death function of BCL2 induced by various stimuli. Related references: PMID:12724406 PMID:9305631
• Localizes to both the nucleus and cytoplasm, participating in diverse cellular processes.
• Isoform 4 is the most abundant and ubiquitously expressed isoform, except in liver, colon, breast, and uterine myometrium; isoform 1 is expressed in ovary and testis; high levels of isoform 4 are observed in leukemia and lymphoma cell lines.
• Involved in the STUB1-mediated proteasomal degradation of ESR1 in response to age-related estradiol decline, thereby promoting neuronal apoptosis in ischemic reperfusion injury (by similarity).
• Keywords: alternative splicing, alternative initiation, chaperone, apoptosis, phosphoprotein, Ubl conjugation, and 3D-structure; indicative of multiple regulatory mechanisms and post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100–200 of human BAG1; consider that the antibody may recognize epitopes in the central region of the protein.
• Validated applications include Western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA.
• Cross-reactivity with human, mouse, and rat is confirmed; this antibody is suitable for studies using rodent models.
• For WB, the predicted molecular weight is approximately 39 kDa; note that endogenous BAG1 has multiple isoforms (e.g., isoform 1 ~50 kDa, isoform 4 ~36 kDa) and may appear as multiple bands depending on the tissue or cell line.
• Validate in the relevant sample system; optimal dilutions and conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research laboratories by providing a comprehensive range of raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents, all backed by technical and sourcing expertise. This BAG1 antibody is suitable for apoptosis, cancer, and chaperone research programs, supporting your assay development from concept to clinic. For bulk supplies or customized sourcing of rare-target raw materials, CamelBio is your reliable partner.