Anti-BAF57/SMARCE1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q969G3

Core Product Specifications and Parameters

Biological Background: BAF57/SMARCE1 Function and Localization

• BAF57 (SMARCE1) is a core subunit of the SWI/SNF ATP-dependent chromatin remodeling complexes, including npBAF (neural progenitors) and nBAF (neuron-specific) complexes. It plays a critical role in altering DNA-histone contacts to regulate transcription.
• Through its association with SWI/SNF complexes, BAF57 is involved in both transcriptional activation and repression of select genes, particularly during neural development. The switch from npBAF to nBAF complexes is essential for differentiation from proliferating neural stem cells to postmitotic neurons.
• BAF57 interacts with nuclear hormone receptors, such as estrogen receptor, coactivating estrogen-responsive promoters in conjunction with SRC/p160 histone acetyltransferases. Additionally, it can bind the CoREST corepressor to repress neuron-specific genes in non-neuronal cells.
• The protein localizes exclusively to the nucleus, consistent with its role in chromatin remodeling and transcriptional regulation.
• Post-translational modifications include phosphorylation, methylation, and ubiquitination, which may modulate its activity and stability. Alternative splicing also generates multiple isoforms.
• BAF57 contains a coiled-coil domain and a DNA-binding region, facilitating protein-protein interactions and chromatin targeting. Keywords such as "Chromatin regulator", "Neurogenesis", and "Disease variant" highlight its importance in neural development and disease, with mutations linked to intellectual disability.

Experimental Guidance and Technical Tips

• For Western blotting, whole-cell lysates from HeLa or HEK293 cells are commonly used to detect endogenous BAF57 at approximately 47 kDa. The KO validation data provided by the manufacturer can serve as a reference for specificity.
• In immunofluorescence/immunocytochemistry, a predominantly nuclear staining pattern is expected. Optimize fixation and permeabilization conditions to preserve nuclear architecture.
• The polyclonal nature of this antibody may enhance capture efficiency in immunoprecipitation experiments. Consider cross-linking the antibody to beads to avoid heavy chain interference in subsequent Western blotting.
• When working with mouse or rat samples, verify cross-reactivity by including positive control lysates and running appropriate secondary antibody controls to rule out nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, technical services, and consulting—supporting diagnostic manufacturers and researchers from concept to clinic. For chromatin remodeling and epigenetics research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing assistance for rare targets. Our one-stop supply chain streamlines your development workflow, ensuring consistent quality and reliability for your assays.