Anti-BAF57/SMARCE1 Rabbit mAb for WB, IF/ICC, IP, ELISA - Q969G3

Core Product Specifications and Parameters

Biological Background: BAF57/SMARCE1 Function and Localization

• BAF57, encoded by the SMARCE1 gene, is a core subunit of SWI/SNF chromatin remodeling complexes, which use ATP hydrolysis to alter DNA-nucleosome contacts and regulate transcription.
• It exists in multiple isoforms due to alternative splicing, contributing to the functional diversity of SWI/SNF complexes.
• BAF57 is integral to the neural progenitor BAF (npBAF) and neuron-specific BAF (nBAF) complexes; a subunit switch from npBAF to nBAF is essential for neuronal differentiation and dendrite growth.
• The protein directly binds DNA and facilitates transcriptional activation of estrogen-responsive promoters by cooperating with SRC/p160 histone acetyltransferases.
• In non-neuronal cells, BAF57 interacts with the CoREST corepressor to silence neuronal-specific genes, highlighting its dual role in activation and repression.
• Subcellularly localized to the nucleus, BAF57 undergoes post-translational modifications including phosphorylation, methylation, and ubiquitination, which may regulate complex assembly and activity.
• Associated keywords include chromatin regulator, neurogenesis, disease variant, and intellectual disability, underscoring its importance in brain development and pathology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 2-100) of human BAF57; this epitope is conserved in mouse and rat, supporting the reported cross-reactivity.
• In Western blot applications, expect a band at approximately 47 kDa; consider using nuclear-enriched lysates for optimal detection.
• For immunofluorescence, anticipate a nuclear staining pattern; validation with appropriate fixation and permeabilization methods is recommended.
• This antibody may be suitable for immunoprecipitation of native SWI/SNF complexes; co-immunoprecipitated proteins should be confirmed by mass spectrometry or specific antibodies.
• ELISA performance can be checked with recombinant BAF57 protein or cell lysates; assay conditions should be empirically optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to this BAF57/SMARCE1 antibody, a key tool for epigenetics and neurobiology investigations. Beyond single reagents, we offer comprehensive IVD raw-material solutions including validated antibody pairs, bulk ancillary reagents, and sourcing of rare targets to accelerate your assay development from concept to clinic.