Anti-Bad Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92934

Core Product Specifications and Parameters

Biological Background: Bad Function and Localization

• Bad (Bcl2-associated agonist of cell death) is a pro-apoptotic member of the Bcl-2 protein family, also known as BBC6, BCL2L8, and Bcl-xL/Bcl-2-associated death promoter.
• It promotes apoptosis by competing for binding to anti-apoptotic Bcl-2 family members (Bcl-X(L), Bcl-2, Bcl-W), thereby freeing pro-apoptotic proteins like BAX to initiate cell death (By similarity).
• Bad functions as a key link between growth factor receptor signaling and the apoptotic machinery, integrating survival signals to regulate cell fate.
• The protein localizes to the mitochondrial outer membrane and cytoplasm, shuttling between these compartments in response to survival factor deprivation.
• Post-translational modifications, including phosphorylation, acetylation, and methylation, dynamically regulate Bad’s pro-apoptotic activity and subcellular localization.
• Bad is ubiquitously expressed across a wide variety of human tissues, consistent with its fundamental role in apoptosis regulation.

Experimental Guidance and Technical Tips

• For western blotting, expect a major band around 18 kDa; validate detection in relevant cell or tissue lysates expressing Bad (e.g., HeLa, Jurkat).
• In immunofluorescence/ICC, consider optimized fixation and permeabilization protocols to preserve mitochondrial versus cytoplasmic staining patterns.
• As a polyclonal antibody raised against an internal peptide, it may recognize multiple epitopes; confirm specificity using appropriate positive and negative controls.
• For ELISA applications, titrate the antibody to determine optimal coating and detection conditions; pairing with a matched capture antibody may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, supporting apoptosis research and assay development. Our Bad rabbit polyclonal antibody provides a reliable tool for detecting Bad in human, mouse, and rat samples, helping diagnostic manufacturers streamline their workflow. Leverage our one-stop sourcing capability to accelerate your concept-to-clinic journey with readily available, high-quality raw materials.