Anti-BACH1 Rabbit Polyclonal Antibody for WB, IP, ELISA - O14867

Core Product Specifications and Parameters

Biological Background: BACH1 Function and Localization

• Transcription regulator BACH1 (BTB and CNC homolog 1, also known as HA2303) is a member of the CNC-bZIP transcription factor family.
• Contains a BTB domain and a basic leucine zipper (bZIP) domain, enabling DNA binding and protein-protein interactions.
• Acts as a context-dependent repressor or activator, binding NF-E2 DNA binding sites and coordinating transcription with MAFK (By similarity).
• Together with MAF, represses transcription of genes under the control of the NFE2L2 oxidative stress pathway, thereby regulating cellular redox balance. Related references: PMID:24035498
• Localizes predominantly to the nucleus, consistent with its function as a transcription regulator.
• Subject to post-translational modifications such as phosphorylation and ubiquitination, which may influence its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-320) of human BACH1, which includes the BTB domain, critical for protein interactions; this region is highly conserved in mouse, supporting cross-reactivity.
• For Western Blot, expect a band at approximately 82 kDa; use whole-cell lysates from human or mouse cell lines and validate with positive controls expressing BACH1.
• In Immunoprecipitation, the polyclonal nature may enhance capture efficiency; optimize antibody and lysate concentrations, and confirm with appropriate controls.
• For ELISA, the antibody may be used for capture or detection; validate with recombinant BACH1 protein or relevant biological samples.
• Consider performing a titration series to determine optimal working concentrations for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, offering this BACH1 polyclonal antibody—ideal for research into transcriptional regulation and oxidative stress pathways. Our extensive portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, bridging the gap from early-stage development to clinical diagnostics.