Anti-BACH1 Rabbit Monoclonal Antibody for WB, ELISA, CHIP - O14867

Core Product Specifications and Parameters

Biological Background: BACH1 Function and Localization

• BACH1 is a transcription regulator protein, also known as BTB and CNC homolog 1, encoded by the BACH1 gene.
• It acts as both a transcriptional repressor and activator, depending on the context, and binds to NF-E2 DNA binding sites.
• Cooperates with MAFK to coordinate transcription activation and repression.
• Together with MAF, represses the transcription of genes under the control of the NFE2L2 oxidative stress pathway. Related references: PMID:24035498
• Localizes predominantly to the nucleus, consistent with its role in transcription regulation.
• Post-translational modifications include phosphorylation and ubiquitination (Phosphoprotein and Ubl conjugation keywords).
• Other relevant keywords include DNA-binding, Activator, Repressor, 3D-structure, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 410-736) of human BACH1. For Western blot, consider the predicted molecular weight of ~82 kDa, though actual mobility may vary due to phosphorylation or other modifications.
• In ELISA, use the recombinant immunogen or purified target protein as a positive control, and validate coating conditions and detection ranges empirically.
• For chromatin immunoprecipitation (CHIP), optimize crosslinking and sonication conditions to preserve native protein-DNA interactions, and confirm antibody specificity with appropriate controls.
• Since the antibody cross-reacts with mouse, it may be suitable for murine models; however, validate detection in your specific sample system.

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