Anti-BACE1 Rabbit Monoclonal Antibody for WB and ELISA - P56817

Core Product Specifications and Parameters

Biological Background: BACE1 Function and Localization

• BACE1 (Beta-secretase 1), also known as ASP2 or memapsin-2, is a type I transmembrane aspartyl protease belonging to the peptidase A1 family, encoded by the BACE1 gene on chromosome 11.
• BACE1 is responsible for the proteolytic processing of the amyloid precursor protein (APP), cleaving at the N-terminus of the Aβ peptide sequence to generate beta-cleaved soluble APP and a C-terminal fragment that is subsequently processed by gamma-secretase to produce amyloid-beta peptides, which are central to Alzheimer's disease pathology.
  Related references: PMID:10656250 PMID:10677483 PMID:20354142
• In addition to APP, BACE1 cleaves other substrates such as CHL1 (close homolog of L1), indicating broader roles in neuronal development and function (By similarity).
• BACE1 localizes to multiple cellular membranes, including the cell surface, Golgi apparatus, trans-Golgi network, endoplasmic reticulum, endosomes (early, late, recycling), lysosomes, and membrane rafts, and is enriched in neuronal projections like axons and dendrites.
• The enzyme is highly expressed in the brain, particularly in the substantia nigra, locus coeruleus, and medulla oblongata, and also shows high expression in the pancreas.
• Post-translational modifications of BACE1 include glycosylation, acetylation, palmitoylation, phosphorylation, and disulfide bond formation; it is synthesized as an inactive zymogen requiring proteolytic activation.
• Alternative splicing generates multiple isoforms, and the protein undergoes ubiquitination and other regulatory modifications, as identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide corresponding to the cytoplasmic domain (amino acids 402-501) of human BACE1, a region unique to BACE1 and not conserved with BACE2. This design may confer specificity to the BACE1 isoform.
• For Western blot (WB), expect detection of full-length BACE1 at approximately 56 kDa. Use appropriate positive controls such as brain tissue lysates or cell lines known to express BACE1. Consider validating specificity with knockout or knockdown samples.
• For ELISA applications, this antibody can be evaluated as either a capture or detection reagent. Optimization of coating conditions and pairing with matched antibodies is recommended.
• Given the reported cross-reactivity with human, mouse, and rat, this antibody is suitable for studies across these species. However, always verify reactivity in your specific experimental system.
• The antibody is shipped on ice bags to maintain stability; store at recommended temperatures upon receipt.

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