Anti-BAAT Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14032

Core Product Specifications and Parameters

Biological Background: BAAT Function and Localization

• BAAT (bile acid-CoA:amino acid N-acyltransferase), also known as BAT, HCHO, BACAT, BACD1, is a key enzyme in bile acid metabolism.
• It catalyzes the conjugation (amidation) of bile acids with glycine or taurine, which promotes bile acid and cholesterol secretion into bile and enhances intestinal lipid absorption. Related references: PMID:12810727, PMID:2037576
• BAAT also functions as an acyl-CoA thioesterase, hydrolyzing long-chain and very long-chain fatty acyl-CoAs to free fatty acids, thus regulating intracellular lipid levels. Related references: PMID:12239217, PMID:12810727, PMID:8034703
• The protein contains serine esterase and acyltransferase domains, and is classified under transferases and hydrolases (UniProt keywords).
• BAAT is predominantly localized in the cytosol and peroxisomes of hepatocytes.
• Expressed in gallbladder mucosa, pancreas, and hepatocytes. Related references: PMID:12810727, PMID:2037576, PMID:23415802
• Genetic variants in BAAT are associated with familial hypercholanemia and other bile acid metabolic disorders (keyword: disease variant).
• Post-translational modifications include phosphorylation (keyword: phosphoprotein), and the enzyme has been identified by direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 309-418) of human BAAT; consider using this antibody in applications where this epitope is accessible.
• For Western blot, a band around 46 kDa is expected; validate with relevant positive controls (e.g., human liver lysates).
• Due to the polyclonal nature, optimization of blocking and dilution conditions may be required to reduce nonspecific binding.
• Cross-reactivity with mouse and rat has been reported; verify in the appropriate model systems if using these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop sourcing solution for IVD raw materials. This high-quality anti-BAAT polyclonal antibody supports research into bile acid metabolism, liver function, and related disorders. With validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents, CamelBio streamlines your procurement from discovery to clinical application.