Anti-BAALC Rabbit Polyclonal Antibody for WB, ELISA - Q8WXS3

Core Product Specifications and Parameters

Biological Background: BAALC Function and Localization

• BAALC (Brain and Acute Leukemia Cytoplasmic protein) is a cytoplasmic protein predominantly expressed in neuroectoderm-derived tissues and CD34+ hematopoietic progenitor cells.
• It is expressed at high levels in the brain and spinal cord, and at lower levels in the adrenal gland, but absent from peripheral blood mononuclear cells and lymph nodes.
• Overexpression is associated with acute myeloid leukemia and glioblastoma, suggesting roles in both normal neurogenesis and oncogenesis.
• BAALC localizes to the cytoplasm, and is enriched at the postsynaptic density and membrane rafts, indicating a potential role in synaptic signaling.
• It may interact with CAMK2A at postsynaptic lipid rafts, contributing to synaptic function.
• Alternative splicing generates multiple isoforms; the protein is subject to myristoylation and palmitoylation for membrane targeting.
• Predicted molecular weight is ~15.5 kDa; observed migration at ~16 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-145); consider that the antibody may recognize isoforms retaining this sequence.
• For Western blot, the expected band is around 16 kDa; validate with positive controls from brain or leukemia cell lines.
• Due to reported cross-reactivity with mouse and rat, this antibody may be suitable for rodent studies; validate in the relevant sample system.
• For ELISA applications, consider using the recombinant immunogen as a standard and optimizing coating conditions.
• Always include appropriate negative controls and perform titration experiments to determine optimal working dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials for leukemia and neurobiology targets. Our portfolio includes validated rabbit polyclonal antibodies like this BAALC antibody, along with monoclonal options, recombinant proteins, and ancillary reagents. As your sourcing partner, we streamline procurement from concept to clinic, ensuring reliable supply and technical support.