Anti-B9D1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UPM9

Core Product Specifications and Parameters

Biological Background: B9D1 Function and Localization

• B9 domain-containing protein 1 (B9D1), also known as MKS1-related protein 1, is encoded by the B9D1 gene (GeneID: 27077).
• B9D1 is a component of the tectonic-like complex, which localizes at the transition zone of primary cilia and acts as a barrier to prevent diffusion of transmembrane proteins between the cilia and plasma membranes.
• It is required for ciliogenesis and sonic hedgehog (SHH) signaling.
• Subcellularly, B9D1 localizes to the cytoplasm, specifically at the cilium basal body and cilium axoneme.
• Mutations in B9D1 are associated with ciliopathies, including Joubert syndrome and Meckel syndrome.
• It is subject to alternative splicing and has been identified through proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to the N-terminal region (aa 1–130) of human B9D1; this region may be conserved across species, supporting the observed cross-reactivity with mouse and rat. Consider sequence alignment when assessing ortholog reactivity.
• For Western blot, B9D1 has a predicted molecular weight of ~23 kDa; ensure appropriate gel percentage and transfer conditions. For immunofluorescence (IF/ICC), B9D1 localizes to ciliary structures, so imaging under high magnification and co-staining with ciliary markers may be beneficial.
• As a polyclonal antibody, batch-to-batch variability is possible; validation in the relevant sample system is recommended.
• Given the involvement in cilia, IHC-P on tissue sections rich in ciliated cells (e.g., kidney, retinal epithelium) may yield informative results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with high-quality raw materials, including validated antibodies for cilia and ciliopathy targets like B9D1. We offer a one-stop supply of optimized monoclonal and polyclonal antibodies, antigen-grade recombinant proteins, and ancillary reagents to support your assay development and biomarker research. For specialized or rare targets, our sourcing team can assist in securing custom reagents to accelerate your transition from concept to clinic.