Anti-B4GALT4 Rabbit Polyclonal Antibody for WB, ELISA - O60513

Core Product Specifications and Parameters

Biological Background: B4GALT4 Function and Localization

• B4GALT4 (Beta-1,4-galactosyltransferase 4) is a 40 kDa glycosyltransferase that catalyzes the transfer of galactose from UDP-Gal to N-acetylglucosamine via a β1-4 linkage, generating terminal N-acetyllactosamine units on glycoproteins and glycosphingolipids. Related references: PMID:12511560 PMID:17690104 PMID:32827291
• In keratan sulfate biosynthesis, B4GALT4 cooperates with B3GNT7 and sulfotransferases to construct and elongate mono- and disulfated disaccharide units. Related references: PMID:17690104
• It is involved in the synthesis of 6-sulfo-sialyl Lewis X (sLex) epitopes on mucin-type O-glycans, which serve as ligands for L-selectin and regulate leukocyte migration. Related references: PMID:12511560
• In neolacto-series glycosphingolipid biosynthesis, B4GALT4 transfers galactose to lactotriaosylceramide to form neolactotetraosylceramide. Related references: PMID:9792633
• B4GALT4 is localized to the Golgi apparatus membrane and can be secreted.
• It is expressed at highest levels in placenta, pancreas, kidney, and heart, and is also found in corneal epithelial cells. Related references: PMID:9792633 PMID:17690104
• The enzyme requires manganese as a cofactor and contains disulfide bonds and glycosylation sites, indicative of its post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 35-344, covering a large portion of the protein, which may enhance antibody reactivity across multiple epitopes.
• For Western blotting, a band around 40 kDa is expected; consider using appropriate loading controls and post-translational modification-aware protocols.
• For ELISA, the antibody may be used for detection or capture; validate with relevant recombinant protein or cell lysates expressing B4GALT4.
• Since B4GALT4 is a Golgi-resident enzyme, subcellular fractionation or enrichment may improve signal detection in cell lines with low endogenous expression.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio offers this anti-B4GALT4 antibody alongside a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement services. Focused on glycobiology and biomarker research, our one-stop supply chain supports diagnostic manufacturers and laboratories from concept to clinic. Contact us to discuss your specific requirements and accelerate your development timeline.