Anti-B4GALT3 Polyclonal Antibody for WB & ELISA - O60512

Core Product Specifications and Parameters

Biological Background: B4GALT3 (Beta-1,4-galactosyltransferase 3) Function and Localization

• B4GALT3 encodes a member of the beta-1,4-galactosyltransferase family, responsible for transferring galactose to N-acetylglucosamine (GlcNAc) residues in glycoconjugates.
• It catalyzes the synthesis of complex-type N-linked oligosaccharides on glycoproteins and the carbohydrate moieties of glycolipids, a key step in glycosylation pathways.
• Catalytic activity: UDP-galactose + N-acetyl-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1→4)-N-acetyl-D-glucosaminylglycopeptide; also acts on neolactotriaosylceramide.
• The enzyme localizes to the Golgi apparatus membrane (stack region), where post-translational modification of secreted and membrane proteins occurs.
• It contains an N-terminal signal-anchor and a single transmembrane domain, orienting the catalytic domain into the Golgi lumen.
• Highest expression is found in placenta, prostate, testis, ovary, intestine, and muscle; also expressed in fetal brain.
• Post-translational modifications include disulfide bonds and N-linked glycosylation, which may influence activity and stability.
• Keywords: Alternative splicing, Glycosyltransferase, Lipid metabolism, Manganese, Metal-binding, Transferase.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 40–150) targets the catalytic domain, likely conserved across human, mouse, and rat, supporting observed cross-reactivity.
• In WB, expect a major band at ~44 kDa; use placenta or testis lysates as positive controls.
• For ELISA, use recombinantly expressed full-length B4GALT3 as a coating antigen; validate with a standard curve.
• Optimize detection for your specific sample type, as endogenous expression varies; include a knockout or knockdown control to confirm specificity.
• Prior to first use, centrifuge the antibody vial briefly and store at recommended temperatures (typically −20°C for long-term storage).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our B4GALT3 rabbit polyclonal antibody supports glycobiology and glycolipid metabolism studies, aligning with your assay development needs. From concept to clinic, CamelBio streamlines sourcing of rare targets and routine raw materials with consistent supply and technical support.