Anti-B4GALT2 Rabbit Polyclonal Antibody for WB, ELISA - O60909

Core Product Specifications and Parameters

Biological Background: B4GALT2 Function and Localization

• Beta-1,4-galactosyltransferase 2 (B4GALT2) catalyzes the transfer of galactose from UDP-galactose to N-acetylglucosamine-containing acceptors, playing a key role in the synthesis of complex-type N-linked oligosaccharides on glycoproteins and the carbohydrate moieties of glycolipids. It can also produce lactose (PubMed:9405390).
  Related references: PMID:9405390
• The enzyme localizes to the Golgi apparatus membrane, specifically the Golgi stack, consistent with its function in late-stage glycosylation processing.
• Tissue expression is broad but weak, with highest levels detected in prostate, testis, ovary, intestine, muscle, and fetal brain.
• Alternative names include Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase, beta4Gal-T2, and N-acetyllactosamine synthase, reflecting its substrate versatility.
• B4GALT2 is a transmembrane protein with a signal-anchor, a single transmembrane helix, and a catalytic domain facing the Golgi lumen. It contains conserved disulfide bonds and a manganese-binding site required for enzymatic activity.
• Post-translational modifications include N-glycosylation, and the protein is classified as a glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 155-271 of human B4GALT2; thus the antibody is expected to recognize an internal region of the protein.
• For Western blot, a specific band at approximately 42 kDa is predicted based on the full-length sequence; however, actual migration may vary depending on post-translational modifications and gel conditions.
• Cross-reactivity has been observed with mouse and rat samples; consider validating the antibody in your species of interest with appropriate controls.
• For ELISA, titrate the antibody to determine an optimal working dilution, and include a recombinant protein standard curve for quantitative assays.
• Due to the polyclonal nature, the antibody may detect multiple epitopes, enhancing sensitivity but requiring careful blocking and optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with a comprehensive, one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and the ability to source rare-target raw materials. This anti-B4GALT2 polyclonal antibody supports glycobiology and glycosylation research, and our team is ready to assist with custom antibody development or supply scale-up to meet your project needs.