Anti-B4GALT1 Rabbit Polyclonal Antibody [KO Validated] for WB and ELISA - P15291

Core Product Specifications and Parameters

Biological Background: B4GALT1 Function and Localization

• B4GALT1 encodes beta-1,4-galactosyltransferase 1, a key glycosyltransferase that catalyzes the transfer of galactose from UDP-galactose to terminal N-acetylglucosamine residues on glycoproteins and glycolipids in the Golgi apparatus, essential for complex N-glycan biosynthesis and antibody Fc glycosylation. Related references: PMID:16157350, PMID:27872474, PMID:29133956, PMID:36280670
• In conjunction with alpha-lactalbumin (LALBA), B4GALT1 forms the lactose synthase complex in the mammary gland, switching its acceptor specificity from GlcNAc to glucose to produce lactose during lactation. Related references: PMID:16157350
• The protein exists in multiple forms: a Golgi-resident transmembrane form, a cell surface form involved in cell–cell and cell–matrix adhesion, and a secreted form capable of extracellular glycan remodeling.
• B4GALT1 is ubiquitously expressed across tissues but shows very low levels in fetal and adult brain, highlighting tissue-specific glycosylation patterns.
• Clinically, mutations in B4GALT1 are associated with congenital disorder of glycosylation type IId (CDG2D), emphasizing its role in human health and disease.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal catalytic domain (aa 45-210), which is conserved across known isoforms. Consider using this region for immunoassay design to ensure broad isoform recognition.
• For Western blotting, expect a primary band near 44 kDa. Glycosylated or processed variants may appear at slightly higher molecular weights; validate in relevant human cell lysates.
• This antibody is KO validated, indicating specific detection of B4GALT1. For critical applications, confirm specificity using B4GALT1 knockout cell lines or tissues.
• ELISA applications may require antibody titration; test optimal coating and detection conditions in the intended sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing platform for IVD raw materials, covering glycosylation, cell adhesion, and lactation biology targets. For B4GALT1 and related pathways, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Leveraging our integrated network, we also assist in procuring rare-target raw materials to accelerate your assay development from concept to clinic.