Anti-B4GALT1 Rabbit Polyclonal Antibody for WB and ELISA - P15291

Core Product Specifications and Parameters

Biological Background: B4GALT1 Function and Localization

• B4GALT1 (Beta-1,4-galactosyltransferase 1) is a membrane-bound enzyme located in the Golgi stacks. It catalyzes the transfer of galactose to N-acetylglucosamine on glycolipids and N-glycans, essential for synthesizing complex-type N-linked glycans. Related references: PMID:16157350, PMID:27872474, PMID:29133956
• The enzyme specifically adds galactose to both antennae of complex N-glycans, enabling mono- and di-galactosylated glycoforms. On immunoglobulin G (IgG), this galactosylation is a prerequisite for subsequent sialylation, which imparts anti-inflammatory properties. Related references: PMID:27872474, PMID:29133956, PMID:36280670
• In the lactating mammary gland, B4GALT1 forms a complex with alpha-lactalbumin (LALBA) to create lactose synthase, switching substrate preference from N-acetylglucosamine to glucose for lactose production. Related references: PMID:16157350
• A cell surface form of B4GALT1 acts as a recognition molecule in cell-cell and cell-matrix interactions, such as during fertilization by binding to ZP3 glycans on the zona pellucida, triggering the acrosome reaction.
• A secreted form has galactosyltransferase activity and may remodel glycans in biological fluids.
• B4GALT1 is ubiquitously expressed but at very low levels in fetal and adult brain.
• Keywords from UniProt include: Glycoprotein, Disulfide bond, Manganese binding, Congenital disorder of glycosylation, Alternative initiation, and Disease variant.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 50–215 within the catalytic domain; thus the antibody may recognize both full-length and secreted forms if present.
• For Western blot, denaturing and reducing conditions are recommended due to disulfide bonds; a band around 44 kDa is expected in human tissue lysates.
• Validate the antibody in positive controls such as liver, kidney, or placental tissue, where B4GALT1 expression is robust; avoid brain if low expression is a concern.
• For ELISA, the antibody may be used as capture or detection reagent; proper antibody titration and antigen coating concentration should be optimized by the end-user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents. For glycobiology and glycosyltransferase research, we provide one-stop access to high-quality antibodies like this anti-B4GALT1 rabbit pAb, along with custom sourcing for rare targets and bulk supply capabilities to accelerate diagnostic development from concept to clinic.