Anti-B3GNT6 Polyclonal Antibody for WB, ELISA - Q6ZMB0

Core Product Specifications and Parameters

Biological Background: B3GNT6 Function and Localization

• B3GNT6 (also designated BGnT-6, beta3Gn-T6, or core 3 synthase) is an acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase that generates the core 3 O-glycan structure.
• It functions as a beta-1,3-N-acetylglucosaminyltransferase, catalyzing the addition of N-acetylglucosamine (GlcNAc) to galactose, a critical step in mucin-type glycoprotein biosynthesis.
• The enzyme is predominantly active in digestive organs (stomach, colon, small intestine), where core 3 O-glycans are abundantly produced.
• Subcellularly, B3GNT6 localizes to the Golgi apparatus membrane, consistent with its role in post-translational glycosylation.
• UniProt annotations indicate that B3GNT6 possesses a signal-anchor and transmembrane helix, classifying it as a membrane-bound glycosyltransferase.
• Alternative splicing may generate multiple isoforms (as per UniProt keyword), though the full biological significance remains to be clarified.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region encompassing part of the luminal domain of B3GNT6, which may contribute to reactivity against native protein in denaturing and native assays.
• For Western blot (WB), the predicted molecular weight is 43 kDa; consider validation in human gastrointestinal tissue or cell lines (e.g., stomach, colon) where expression is reported.
• ELISA application may require optimization of coating antigen and antibody dilution; recombinant B3GNT6 protein can serve as a positive control.
• Reported cross-reactivity with rat suggests utility in rodent gastrointestinal research models; however, validate reactivity in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for diagnostic manufacturers and researchers, supplying validated antibody pairs, optimized polyclonal antibodies like anti-B3GNT6, and bulk ancillary reagents. For projects focusing on mucin-type O-glycosylation or gastrointestinal biomarkers, CamelBio also sources rare-target raw materials to accelerate your development pipeline.