Anti-B3GALT4 Rabbit Polyclonal Antibody for WB, ELISA - O96024

Core Product Specifications and Parameters

Biological Background: B3GALT4 Function and Localization

• B3GALT4 is a beta-1,3-galactosyltransferase (also known as Gal-T2) that plays a key role in ganglioside biosynthesis, specifically catalyzing the transfer of galactose to N-acetylgalactosamine in the formation of GM1, GD1b, and GA1 gangliosides.
• This enzyme is a type II membrane protein localized to the Golgi apparatus membrane, consistent with its function in glycan modification within the secretory pathway.
• B3GALT4 exhibits a single-pass membrane topology with a signal-anchor sequence and a large catalytic domain oriented to the Golgi lumen.
• It is highly expressed in heart, skeletal muscle, and pancreas, with lower expression in brain, placenta, kidney, liver, and lung, suggesting tissue-specific roles in ganglioside synthesis.
• As a glycosyltransferase, B3GALT4 is involved in lipid metabolism and glycoprotein biosynthesis, contributing to cell surface glycosylation patterns.
• UniProt classifies it as a transferase and lists additional keywords such as membrane, Golgi apparatus, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 80–270, encompassing the lumenal catalytic domain, which should detect full-length endogenous B3GALT4 in WB under reducing conditions.
• The predicted molecular weight is 42 kDa (from supplier data), while UniProt calculates 41.5 kDa; consider possible post-translational modifications or gel system variations during validation.
• For ELISA, direct coating of recombinant protein or cell lysates is suitable; cross-reactivity with mouse enables utilization in mouse model studies.
• Validate the antibody in your specific sample system using appropriate positive controls (e.g., heart or pancreas lysates) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing solutions, including validated antibody pairs, optimized polyclonal antibodies like this B3GALT4 pAb, and bulk ancillary reagents for diagnostic development. With expertise in ganglioside and glycobiology research, we support your transition from concept to clinic with a reliable supply chain.