Anti-B-Raf Rabbit pAb for WB, IHC-P, ELISA - P15056

Core Product Specifications and Parameters

Biological Background: B-Raf Function and Localization

• B-Raf (BRAF) is a serine/threonine-protein kinase, also known as proto-oncogene B-Raf, p94, or v-Raf murine sarcoma viral oncogene homolog B1.
• It functions as a central transducer of mitogenic signals from the cell membrane to the nucleus by phosphorylating MAP2K1 (MEK), thereby activating the MAP kinase signal transduction pathway. Related references: PMID:21441910 PMID:29433126
• B-Raf also phosphorylates PFKFB2, linking it to metabolic control. Related references: PMID:36402789
• It may contribute to postsynaptic responses in hippocampal neurons. Related references: PMID:1508179
• Subcellular localization includes the nucleus, cytoplasm, and cell membrane.
• Highly expressed in brain and testis tissues.
• Contains a zinc-finger domain and undergoes multiple post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination.
• Germline mutations in BRAF are associated with developmental disorders (e.g., cardio-faciocutaneous syndrome, Noonan syndrome) and various cancers; it is a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 350–449 within the kinase domain, suggesting potential recognition of both wild-type and common mutant B-Raf; however, binding to specific variants should be validated in the relevant sample system.
• For Western blot, the predicted molecular weight is approximately 84 kDa; consider using appropriate molecular weight markers and that post-translational modifications may cause slight shifts.
• For immunohistochemistry (paraffin-embedded sections), optimization of antigen retrieval and antibody dilution is recommended to achieve specific staining.
• Reported cross-reactivity with mouse and rat B-Raf should be confirmed individually in each experimental setup.

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CamelBio offers diagnostic manufacturers and laboratories a comprehensive, one-stop supply of IVD raw materials, including validated anti-B-Raf antibodies like this one. We support kinase signaling research and oncology assay development with optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and access to rare-target raw materials. Contact us to efficiently bridge your sourcing needs from concept to clinic.